Just Stop Oil protesters interrupted play twice at Wimbledon by throwing orange-coloured confetti and jigsaw pieces on to court 18.
The contest between Grigor Dimitrov and Sho Shimabukuro was targeted by two protesters.
A third protester then targeted Katie Boulter’s match against Daria Saville. Two men and a woman were arrested.
Environmental protest group Just Stop Oil has targeted various sporting events, including an Ashes Test.
Followers of the group – which calls for the government to halt all new oil, gas and coal projects – also disrupted the rugby union Premiership final and the World Snooker Championship.
In a statement after the first episode, the All England Club (AELTC) said: “Following an incident on court 18, two individuals have been arrested on suspicion of aggravated trespass and criminal damage and these individuals have now been removed from the grounds.”
A man and woman had run on to the court, throwing confetti and jigsaw pieces from a Wimbledon ‘Centre Court View’ jigsaw puzzle box on to the grass. The man sat down on court 18 before he was removed.
A couple of hours later another man ran on to the same court, where British number one Boulter had just resumed her rain-interrupted first-round match against Australian Saville.
The crowd booed the protesters with some heard shouting “get off”.
Following the breaks in play, a Wimbledon shop worker told BBC Sport they had stopped selling jigsaws for now.
Just Stop Oil said in a statement its supporters had thrown “environmentally friendly orange confetti glitter and jigsaw pieces” and that “play was briefly delayed whilst marshals picked up the pieces”.
Home secretary Suella Braverman condemned the actions of the protesters as “selfish” and “unacceptable”, and said she had chaired a meeting at Downing Street with representatives of various sports organisations to try to “prevent further disruptions”.
Culture Secretary Lucy Frazer added: “We must protect the right to peaceful protest, but that does not give licence to a vocal minority to spoil events that millions of us enjoy.”
The meeting followed a spate of protests at high-profile sporting events, with Just Stop Oil disrupting the second Ashes cricket test at Lord’s last week, attempting to spread orange powder on the wicket.
